RESUMO
Acute posttraumatic period of severe maxillofacial trauma in case of hyperbaric oxygenation therapy is followed by expressed activation of lipide-peroxide-oxidation (LPO) in erythrocytic membrane on the background of significant reduction of the substrate for fermentative antioxidation system (AOS). Imbalance in the system LPO:AOS is followed by expressed structural reorganization in erythrocytic membrane and build-up extraerythrocytic haemoglobin in plasma. Klonidine promotes LPO activity reduction and prevents depletion of AOS fermentative capacity in membrane that is followed by normalization of structural-functional interrelations in membrane and reduction of the level of extraerythrocytic haemoglobin in plasma.
Assuntos
Agonistas alfa-Adrenérgicos/uso terapêutico , Clonidina/uso terapêutico , Traumatismos Craniocerebrais/diagnóstico , Traumatismos Craniocerebrais/tratamento farmacológico , Membrana Eritrocítica/química , Peroxidação de Lipídeos , Agonistas alfa-Adrenérgicos/farmacologia , Adulto , Catalase/análise , Membrana Eritrocítica/efeitos dos fármacos , Feminino , Hemoglobinas/análise , Humanos , Peroxidação de Lipídeos/efeitos dos fármacos , Masculino , Malondialdeído/análise , Superóxido Dismutase/análise , ViscosidadeRESUMO
For acute posttraumatic period of heavy combined cranium-facial trauma (CFT) considerable activation of peroxide lipids oxidation in the liquor is typical beginning from the 1st day of posttraumatic period on the background of speedy and drastic depletion of fermentative and low-molecular antioxidant liquor system (in spite of introduction of antioxidants particularly a-tocopherol acetate). Non-adequate functioning of the system of antioxidant defense on the background of free radical activity splash can be considered as breakdown of the process of adaptive reaction forming. It leads to weighting the course of posttraumatic period of heavy combined cranium-facial trauma and its outcome as a whole.
Assuntos
Traumatismos Craniocerebrais/líquido cefalorraquidiano , Traumatismos Faciais/líquido cefalorraquidiano , Malondialdeído/líquido cefalorraquidiano , Traumatismo Múltiplo/líquido cefalorraquidiano , Estresse Oxidativo/fisiologia , Superóxido Dismutase/líquido cefalorraquidiano , alfa-Tocoferol/líquido cefalorraquidiano , Adulto , Biomarcadores/líquido cefalorraquidiano , Ceruloplasmina/líquido cefalorraquidiano , Feminino , Seguimentos , Humanos , Peroxidação de Lipídeos/fisiologia , Masculino , Prognóstico , Índices de Gravidade do TraumaRESUMO
We studied the effect of single intravenous injection of perftoran on the intensity of the initial stages of lipid peroxidation and structural and functional characteristics of erythrocyte membranes (cell model). Perftoran slightly activated the initial stages of lipid peroxidation and optimized structural and functional characteristics of erythrocyte membranes.
Assuntos
Membrana Eritrocítica , Fluorocarbonos/farmacologia , Peroxidação de Lipídeos , Animais , Substitutos Sanguíneos/metabolismo , Substitutos Sanguíneos/farmacologia , Catalase/metabolismo , Membrana Eritrocítica/química , Membrana Eritrocítica/efeitos dos fármacos , Membrana Eritrocítica/metabolismo , Fluorocarbonos/metabolismo , Hemoglobinas/metabolismo , Masculino , Ratos , Ratos Wistar , Superóxido Dismutase/metabolismoAssuntos
Circulação Cerebrovascular/efeitos dos fármacos , Clonidina/uso terapêutico , Traumatismos Craniocerebrais/tratamento farmacológico , Oxigenoterapia Hiperbárica , Fármacos Neuroprotetores/uso terapêutico , Adulto , Catalase/metabolismo , Clonidina/administração & dosagem , Terapia Combinada , Traumatismos Craniocerebrais/sangue , Traumatismos Craniocerebrais/fisiopatologia , Membrana Eritrocítica/efeitos dos fármacos , Membrana Eritrocítica/enzimologia , Membrana Eritrocítica/metabolismo , Feminino , Humanos , Peróxidos Lipídicos/metabolismo , Masculino , Fármacos Neuroprotetores/administração & dosagem , Superóxido Dismutase/metabolismo , Índices de Gravidade do Trauma , Resultado do TratamentoRESUMO
Acute posttraumatic period of severe craniocerebral trauma is associated with sharp activation of LPO processes and rapid exhaustion of antioxidant enzymes and especially low-molecular-weight antioxidant system in the liquor. This leads to the development of severe oxidative stress and failure of adaptation processes during the early posttraumatic period.
Assuntos
Lesões Encefálicas/líquido cefalorraquidiano , Peroxidação de Lipídeos , Fármacos Neuroprotetores/uso terapêutico , Adulto , Lesões Encefálicas/tratamento farmacológico , HumanosRESUMO
Central and peripheral bloodflow before and after intramuscular and intravenous injections of 0.9% NaCl (control groups, n=20) and perfluothane (n=20) in a dose of 5 ml/kg was studied in 40 Wistar rats. Dopplerographic study of the bloodflow was carried out on a diagnostic device Biomedical AU-5 (Italy). The study showed that intramuscular injections of both 0.9% NaCl and perfluothane did not lead to a significant increase of the bloodflow, but perfluothane caused a significantly greater increase of both arterial and venous bloodflow in the injection zone in comparison with the control. Intravenous injection of perfluothane increased significantly the systemic and local bloodflow in comparison with the baseline level and control, while total peripheral resistance was decreased.
Assuntos
Circulação Sanguínea/efeitos dos fármacos , Substitutos Sanguíneos/administração & dosagem , Fluorocarbonos/administração & dosagem , Animais , Velocidade do Fluxo Sanguíneo/efeitos dos fármacos , Substitutos Sanguíneos/farmacologia , Fluorocarbonos/farmacologia , Injeções Intramusculares , Injeções Intravenosas , Masculino , Ratos , Ratos Wistar , Fluxo Sanguíneo Regional/efeitos dos fármacos , Cloreto de Sódio/administração & dosagem , Cloreto de Sódio/farmacologia , Ultrassonografia DopplerRESUMO
Acute posttraumatic stage of severe craniocerebral trauma was characterized by considerable activation of lipid peroxidation and exhaustion of the antioxidant enzyme system. Oxidative modification of membranes was accompanied by structural and functional changes. The antihypoxic effect of clonidine was realized directly in the lipid phase of membranes. This preparation inhibited lipid peroxidation and activated antioxidant enzymes, which prevented the development of structural and functional changes in membranes.